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KMID : 0380020060210050347
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 5 p.347 ~ p.352
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The Early Detection of the Gram Positive Bacteria using Sonification and Fluorescent Dye in the Field
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Ha Yeon-Chul
Choi Ki-Bong
Choi Jung-do
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Abstract
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This study was carried out to establish the optimum condition for cell disruption with a sonificator in the detection of the gram positive bacteria, Bacillus globigii and Streptococcus epidermidis for the purpose of developing automatic fluorometer. The efficiency of sonificator on the Bacillus globigii and Streptococcus epidermidis disruption differed greatly according to the diameter of sonificator probe tip. The larger sonificator probe diameter showed greater disruption. Bacillus globigii was more disruptive than Streptococcus epidermidis. Sonificator probe of the 13 mm diameter was the most efficient one when sample was sonificated for 20 seconds. The detection limits of Bacillus globigii and Streptococcus epidermidis were 105 CFU/㎖ and 5 × 105 CFU/㎖ respectively when samples were sonificated for 20 seconds with a sonificator probe of 13 mm diameter.
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KEYWORD
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Sonification, disruption, fluorometer, detection
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